Use of DNA and peptide nucleic acid molecular beacons for detection and quantification of rRNA in solution and in whole cells.

Whole Cells Quantification of rRNA in Solution and in Molecular Beacons for Detection and Use of DNA and Peptide Nucleic Acid

Cellular Morphology and Immunologic Properties of Escherichia coli Treated With Antimicrobial Antisense Peptide Nucleic Acid

  Background & Objectives: Antisense peptide nucleic acids (PNA) that target growth essential genes show potent bactericidal properties without cell lysis. We considered the possibility that whether PNA treatment influence the bacteria total nucleic acids content and apply approach to develop a new delivery system to Dendritic cells (DCs). DCs are the most potent antigen presenting cells in th...

Evaluation of microfluidic biosensor development using microscopic analysis of molecular beacon hybridization kinetics.

Molecular beacons, oligonucleotide probes that fluoresce upon hybridization to a target nucleic acid, can be used in microfluidic devices to detect and quantify nucleic acids in solution as well as inside bacterial cells. Three essential steps towards the development of such devices as integrated microfluidic biosensors using molecular beacons were investigated in the present study. First, expe...

Specific detection of Shigella sonnei by enzyme-linked aptamer sedimentation assay

Development of potent new anti-Shigella agents for rapid and specific detection and treatment is of great importance. Aptamers, nucleic acid oligomers capable of specific binding to a wide range of non-nucleic acid targets, may be of value for this purpose. In the present study, we used a Systematic Evolution of Ligands by Exponential enrichment (SELEX) process to select DNA aptamers that b...

Optimization of the Analysis of Almond DNA Simple Sequence Repeats (SSRs) Through Submarine Electrophoresis Using Different Agaroses and Staining Protocols

Simple sequence repeat (SSR markers or microsatellites), based on the specific PCR amplification of DNA sequences, are becoming the markers of choice for molecular characterization of a wide range of plants because of their high polymorphism, abundance, and codominant inheritance. Different methods have been used for the analysis of the SSR amplified fragments being submarine agarose electropho...